Journal: Radiation research

Article Title: Transcript Analysis for Internal Biodosimetry Using Peripheral Blood from Neuroblastoma Patients Treated with 131 I-mIBG, a Targeted Radionuclide

doi: 10.1667/RR14263.1

Figure Lengend Snippet: Selected Transcripts of Interest

Article Snippet: The DNA repair domain was represented by XPC and DDB2 . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Gene Name Primer no. GAPDH Glyceraldehyde 3-phosphate dehydrogenase H202758991_g1 CDKN1A Cyclin-dependent kinase inhibitor 1A (p21) Hs0035578_m1 FDXR Ferrodixin reductase Hs00244586_m1 GADD45A Growth arrest and DNA-damage-inducible alpha Hs00169255_m1 DDB2 Damage-specific DNA binding protein 2 (XPE) Hs03044953_m1 XPC Xeroderma pigmentosum, complementation group C Hs00190295_m1 MDM2 E3 ubiquitin protein ligase Hs00234753_m1 BCL2 B-cell CLL/lymphoma 2 Hs99999018_m1 BCLXL BCL2-like 1 Hs00236329_m1 BAX BCL2-associated X protein Hs99990001_m1 STAT5B Signal transducer and activator of transcription 5B Hs00273500_m1 Open in a separate window Selected Transcripts of Interest Generation of cDNA and Real-Time PCR For RT-PCR analysis, 200 ng of RNA was converted to single-strand cDNA using the High Capacity cDNA archive kit (Applied Biosystems ® , Foster City, CA).

Techniques: Binding Assay, Ubiquitin Proteomics

Journal: Radiation research

Article Title: Transcript Analysis for Internal Biodosimetry Using Peripheral Blood from Neuroblastoma Patients Treated with 131 I-mIBG, a Targeted Radionuclide

doi: 10.1667/RR14263.1

Figure Lengend Snippet: Box-whisker plots show the modulation of selected transcripts over time. This collection of plots shows the changes in gene expression at each time interval after 131I-mIBG treatment. The x-axis shows the time points sampled (0–96 h), while the y-axis shows the transformed log2 fold change of expression levels. The transcripts BAX, CDKN1A, DDB2, FDXR, GADD45A, MDM2, STAT5B and XPC show time-dependent upregulation while BCLXL shows time-dependent downregulation (paired Student's t test vs. 0 h after treatment, ***P < 0.001). BCL2 did not have a time-dependent response to radiation.

Article Snippet: The DNA repair domain was represented by XPC and DDB2 . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Gene Name Primer no. GAPDH Glyceraldehyde 3-phosphate dehydrogenase H202758991_g1 CDKN1A Cyclin-dependent kinase inhibitor 1A (p21) Hs0035578_m1 FDXR Ferrodixin reductase Hs00244586_m1 GADD45A Growth arrest and DNA-damage-inducible alpha Hs00169255_m1 DDB2 Damage-specific DNA binding protein 2 (XPE) Hs03044953_m1 XPC Xeroderma pigmentosum, complementation group C Hs00190295_m1 MDM2 E3 ubiquitin protein ligase Hs00234753_m1 BCL2 B-cell CLL/lymphoma 2 Hs99999018_m1 BCLXL BCL2-like 1 Hs00236329_m1 BAX BCL2-associated X protein Hs99990001_m1 STAT5B Signal transducer and activator of transcription 5B Hs00273500_m1 Open in a separate window Selected Transcripts of Interest Generation of cDNA and Real-Time PCR For RT-PCR analysis, 200 ng of RNA was converted to single-strand cDNA using the High Capacity cDNA archive kit (Applied Biosystems ® , Foster City, CA).

Techniques: Whisker Assay, Gene Expression, Transformation Assay, Expressing

Journal: Radiation research

Article Title: Transcript Analysis for Internal Biodosimetry Using Peripheral Blood from Neuroblastoma Patients Treated with 131 I-mIBG, a Targeted Radionuclide

doi: 10.1667/RR14263.1

Figure Lengend Snippet: Multiple Regression Analysis Results for Gene Expression Based on Dose and Time

Article Snippet: The DNA repair domain was represented by XPC and DDB2 . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Gene Name Primer no. GAPDH Glyceraldehyde 3-phosphate dehydrogenase H202758991_g1 CDKN1A Cyclin-dependent kinase inhibitor 1A (p21) Hs0035578_m1 FDXR Ferrodixin reductase Hs00244586_m1 GADD45A Growth arrest and DNA-damage-inducible alpha Hs00169255_m1 DDB2 Damage-specific DNA binding protein 2 (XPE) Hs03044953_m1 XPC Xeroderma pigmentosum, complementation group C Hs00190295_m1 MDM2 E3 ubiquitin protein ligase Hs00234753_m1 BCL2 B-cell CLL/lymphoma 2 Hs99999018_m1 BCLXL BCL2-like 1 Hs00236329_m1 BAX BCL2-associated X protein Hs99990001_m1 STAT5B Signal transducer and activator of transcription 5B Hs00273500_m1 Open in a separate window Selected Transcripts of Interest Generation of cDNA and Real-Time PCR For RT-PCR analysis, 200 ng of RNA was converted to single-strand cDNA using the High Capacity cDNA archive kit (Applied Biosystems ® , Foster City, CA).

Techniques: Gene Expression